README for Figure S1f.csv 
*** This file contains the raw data obtained on DNA, DNA-MvaT and DNA-MvaT-gp4 complexes using bridging assay experiments represented in Figure S1f of 

Article: Novel anti-repression mechanism of H-NS proteins by a phage protein
Authors: Ben Bdira, Erkelens, Qin, Volkov, Lippas, Bowring, Boyle, Ubbink, Dove, Dame 
Journal: Nucleic Acids Research 

Corresponding authors: fredjbdira@gmail.com and rtdame@chem.leidenuniv.nl 

Legend Figure S1: (a) Electrophoretic mobility shift assay (EMSA) of a 200bp DNA substrate at different
concentrations of MvaT wild type (upper panel), MvaT F36D/M44D (middle panel) and gp4 (lower
panel). (b)&(c) Schematic representations of the TPM and bridging assays, respectively, of the
experimental data shown in figure (1f,g). (d) Time dependent bridging assay of DNA-MvaT-DNA
complexes. The blue curve represents the kinetics of MvaT-DNA bridge formation without adding gp4.
The red and pink curve represent the kinetics of MvaT-DNA bridge formation with gp4 introduced at
180 s and 1200 s, respectively. The grey curve represents the introduction of gp4 at the beginning of
the assay (t = 0 s). Error bars represent the standard deviation from at least two independent
measurements. The assays were performed in the presence of 2.4 μM MvaT and 2.4 μM gp4 (1: molar
ratio). The orange point represents a control measurement without proteins at 1200s of incubation. (e)
Dynamic light scattering profiles of 100 μM of MvaT wild type in the absence (left panel) and presence
of 500 μM gp4 (right panel) in 20mM Tris-HCl, pH 8, 300 mM KCl. Note the increase in particle size
of MvaT upon complex formation with gp4. (f) Effect of the gp4 C-terminal helix (residues 30-46) on
MvaT DNA bridging activity.
.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S1f. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of MvaT added to sample in micromolar
Column E: Amount of gp4 added to sample in micromolar 
Column F: Counts per minutes 
Column G: DNA recovery (%)

Columns B until E indicate the contents of the sample. Column F indicates the radioactivity of the sample. Column G indicates the DNA recovery, which is calculated by subtracting the value in column F of the control (sample minus bait DNA) from the sample itself. This value is then divided by the value in column F of sample 1 and multiplied by 100%. 